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Substrate may mean:
* Substrate (aquarium), the material used in the bottom of an aquarium
* Substrate (biochemistry), a molecule which is acted upon by an enzyme
* Substrate (materials science), the material on which a process is conducted
* Substrate (biology), the natural environment in which an organism lives, or the surface or medium on which an organism grows or is attached
* Substrate (chemistry), the catalytic material upon which chemical species react
* Substrate (marine biology), the earthy material that exists in the bottom of a marine habitat, like dirt, rocks, sand, or gravel
* Substrate (printing), the base material that images will be printed onto
* The stratum on which another geologic stratum lies
Substrate may also refer to:
* Wafer (electronics), material upon which semiconductor devices are fabricated
* Printed circuit board (electronics), or the electrically insulating portion of a PCB structure
* Substratum, in linguistics, a language that influences but is supplanted by a second language
* Neural substrate, in neuroscience, the set of brain structures that underlies a specific behavior or psychological state